User:Karmella Haynes/Notebook/Polycomb project/2010/07/19

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07/19/10

  • ✓ Nanostring assay: set up 10 cm plates -/+ dox for KAH126-1, 128-8.3, 129-4, 132-8, 154-2, 156-5, 157-1, 158-4, 159-2, FTRx; check reagents for QIAshredder and RNeasy Mini protocols
  • ✓ Cytology: glass-bottom 24-well plate; -/+ dox for KAH126-1, 128-8.3, 129-4, 132-8, 154-2, 156-5, 157-1, 158-4, 159-2
  • ✓ Senescence assay (replicate 4-day assay): 6-well plates, -/+ dox for KAH126-1, 128-8.3, 129-4, 132-8, 154-2, 156-5, 157-1, 158-4, 159-2, FTRx, and DMSO/rotenone FTRx
  • ✓ Cell culture: split confluent plates 1:10 for back-up cultures
  • ✓ Stable lines: pick colonies from KAH130 and 131 plates (24 each); use 24-well plates