User:Karmella Haynes/Notebook/Polycomb project/2010/07/15

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07/15/10

  • ✓ Senescence assay: flow cytometry on 8-day -/+ dox samples



Senescence assay
> Samples:
1,2. KAH126-1 -/+ dox
3,4. KAH126-3 -/+ dox
5,6. KAH132-8 -/+ dox
7,8. KAH154-2 -/+ dox
9,10. KAH128-8.3 -/+ dox
11,12. KAH129-4 -/+ dox

> Treat w/ 1.5 μg/mL C12-FDG/ 37°C/ 45 min. before harvesting for flow cytometry
> Wash once with DPBS and resuspend in 0.5 mL DPBS
> Spin through filter-cap