User:Karmella Haynes/Notebook/Polycomb project/2010/06/27

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06/27/10

  • ✓ Transfection (H3me reporter/ FTRx cells): refresh medium (U2OS plain)
  • ✓ Split cultures: 130-7, 127-4, 128-8.3 1:10 (10 cm dishes)
  • ✓ Western: 6-well plates for 130-7, 127-4, 128-8.3 dox -/+
  • ✓ H3me sensor lines (old transfection): expand FTRx lines to 12-well plates (2-wells each, 1/2 split); transfer 126-1 lines to new 24-well plates (2-wells each, 1/2 split, dox -/+)