User:Karmella Haynes/Notebook/Polycomb project/2010/05/27

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05/27/10

  • ✓ Thaw cells: KAH130-2
  • ✓ Senescence assay: order rotenone (Sigma) and C12-FDG (Invitrogen)
  • ✓ Frozen stocks: Pc-ATF neg ctrls (5 lines frozen today, 4 slow growers remaining)
  • ✓ Growth assay: split cell lines 1:10 in 10 cm plates; 1 +ab's, 1 plain med., 1 plain med. + 2μg/mL dox