05/09/10
- ✓ Pc-ATF neg ctrl lines: screen for RFP; choose 6 col's per line
- ✓ Misc cell culture: humidifying water was dried up in the incubator; lost 126-4; split others (2x 10cm plates, one for frozen stocks)
- ✓ KAH126-4: thaw new stock (last one!)
Pc-ATF neg ctrl lines RFP screen
> 96-well plates, 32 wells per line
> Screen for nuclear dox-inducible expression of RFP; RFP+ in dox+ and RFP- in dox-
> Key (dox+/dox-)
--> - = no expression
--> + = weak/ non-uniform expression
--> ++ = low or medium uniform expression
--> +++ = very strong expression (uniform or non-uniform)
--> ? = odd morphology/ strange RFP localization
Line |
1 |
2 |
3 |
4 |
5 |
6
|
KAH154 |
++/- |
++/- |
++/- |
+++/+ |
++/- |
++/-
|
KAH155 |
++/+ |
++/- |
++/+ |
++/+ |
+++/+ |
++/-
|
KAH156 |
+++/- |
+++/+ |
++/- |
++/- |
++/- |
+++/+
|
KAH157 |
++/- |
++/- |
++/- |
++/- |
++/- |
++/-
|
KAH158 |
++/- |
++/- |
++/- |
++/- |
++/- |
+/-
|
KAH159 |
++?/- |
++?/- |
++?/- |
++?/- |
+/- |
+/-
|
--> Note: KAH159 clones with ++ expression show a strange single bright dot of RFP, plus medium level RFP expression throughout the nucleus. Nuclear protein aggregate?
--> Tomorrow: transfer dox- cells to 24-well plates
|