User:Karmella Haynes/Notebook/Polycomb project/2010/04/28

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  • Minipreps: Used wrong concentration of Amp for colonies. Regrow picked plate and grow two new cultures
  • ✓ Cultures: FlpE (2 per colony, 4 total) (use 20 μg/mL Amp/LB)
  • ✓ KAH126 and 132 starter cultures: refresh 20% FBS (some aren't growing well)
  • ✓ KAH154-159/MV2 stables: split non-clonal back-up stocks in 6-well plates (selective medium)

Note: Pc-ATF + H3me reporter stables: not growing well (back-up stocks also dying); prepare to repeat with less hygro (redo kill-curve?)