User:Karmella Haynes/Notebook/Polycomb project/2010/04/26

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04/26/10

  • ✓ Re-transformation: FlpE plasmid (use 20 and 10 μg/mL Amp)
  • ✓ Primer order: mCherry internal for RT-PCR of single mCherry fusions



Order primers
--> Product length = 100 bp

  1. mCh f1 5'-CCCGCCGACATCCCCGACTA
  2. mCh r1 5'-GGGTCACGGTCACCACGCC
  3. mCh f2 5'-AGCTGCGCGGCACCAACTTC
  4. mCh r2 5'-GCGCCGTCCTCGGGGTACAT