User:Karmella Haynes/Notebook/Polycomb project/2010/04/25

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04/25/10

  • ✓ Pc-ATF + H3me Reporter lines: colony plates



Pc-ATF + H3me Reporter lines: colony plates
> For each line make 1 plate: KAH126, ~2x105 cells; KAH130-2, 132-8, ~1x105 cells
> 25 mL 300 μg/mL hygro, 15 μg/mL blast, 0.5 μg/mL puro
> Also make 6-well back-up plate for all transfections (~3x105 cells in selection medium)