User:Karmella Haynes/Notebook/Polycomb project/2010/04/19

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04/19/10

  • ✓ KAH131-9: Split 96-well cultures to 2 new plates, dox-induce one plate
  • ✓ KAH131-9: Pick colonies for new 96-well set
  • ✓ Transfection prep: U2OS plain, 12-well plate (1x105/ well); for testing H3me reporters (+/- KAH60)



KAH131-9 colonies
> 4/20/10: No RFP+ in any dox+ wells
> Added 1μg/mL dox to remaining colony plate to see if there is any expression at all
> 4/21/10: saw no expression on entire plate. Discard KAH131-9 cultures and try again later.