User:Karmella Haynes/Notebook/Polycomb project/2010/04/02

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04/02/10

  • ✓ KAH131-9: seed colony selection plates; large gridded plates; 25 ml 0.5 puro, 200 zeo, 15 μg/mL blast, 10% FBS (ts), 1% p/s; diln. 1 = ~3x105, diln. 2 = ~1.5x105; also make 2 back-up cultures (1x 10 cm plain medium, 1x 10 cm ab medium)