User:Karmella Haynes/Notebook/Polycomb project/2010/03/28

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03/28/10

  • ✓ Plate cells for microscopy: KAH126-1, 130-2, 132-8, U2OS plain (H3, H3K27me3, H3K9me3 IF); ~2x105/well, 6 wells/line, in U2OS plain medium + 1 μg/mL dox
  • ✓ Split cells: KAH126-1, 130-2, 132-8; 1x Plain medium 10 cm plate, 1x zeo/blast/puro 10 cm plate
  • ✓ KAH128-8: split 96-well to 2 new plates; add 1 μg/mL dox to duplicate set for RFP screening