User:Karmella Haynes/Notebook/Polycomb project/2010/03/09

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03/09/10

  • ✓ Chromatin IP: cross-link KAH127-4, 128-8, 130-2, 132-8
  • ✓ Dilution plates for selecting RFP+ colonies w/ improved expression: KAH131-9, KAH128-8

Chromatin IP: Cross-linking
> Check for RFP expression
> Qingqing's dual cross-linking protocol (DMA and formaldehyde cross-linking)
> Use 5x 15 cm plates (~2x107 cells/ plate)
> Freeze cells in liq. nitorgen and store at -80°C