User:Karmella Haynes/Notebook/Polycomb project/2009/12/30

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12/29/09

  • ✓ Polycomb-ATF lines: Pick 12 RFP+ colonies per cell line, transfer to 1 ml medium (McCoy's 5A, 10% FBS t.s., 1% P/S, 0.5 μg/mL puro, 200 μg/mL zeo, 15 μg/mL blast)