User:Karmella Haynes/Notebook/Polycomb project/2009/12/23

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12/23/09

  • ✓ Stable Polycomb-ATF lines: expand to selection plates (15 cm grided dishes; 25 mL total; 1 μg/mL puromycin, 10% tet-screened FBS, p/s); use entire amount of cells from each 6-well dish