User:Karmella Haynes/Notebook/Polycomb project/2009/11/30

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11/30/09

  • ✓ Split cells: HEK293 (1x 1:10 flask; 2x 24-well plates)

Transfection plates
> #4 Luc HEK293 cells
> 2x 24-well plates, 1x105 cells/well
--> Dilute 5 mL of 10 mL (~90% confluent) suspension in 50 mL total medium
--> Use phenol red-, a.b.- medium
> Make 1:10 back-up stock in regular medium