User:Karmella Haynes/Notebook/Polycomb project/2009/11/20

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

11/20/09

  • RFP/ Luciferase assay, HEK293 transfectants

RFP/ Luc expression
> Transient transfectants from 11/19/09
> Detach cells by pipetting in growth medium (1 mL)
> Aliquot 100 μL cells (3 wells per samples 50 - 400 ng plasmid) into
---> A black walled, clear bottom 96-well plate (mCherry reads)
---> A white opaque 96-well plate (luciferase assay)
> Use Plate reader protocol "Jake mCherry, OD" for RFP
> Add 100 μL Bright-GLO luciferase assay buffer; R.T. ~ 2 min.
---> Use plate reader protocol "Joe luc"