User:Karmella Haynes/Notebook/PcTF Genomics/2014/09/05

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09/05/14

  • Transformations: FUW lenti plasmids



Transformations: FUW lenti plasmids
Note: KAH015 is amp/zeo resistant (8402 bp)

  1. KAH160/KAH015 - full length hPcTF (1147 bp insert)
  2. KAH165/KAH015 - deleted VP64 (991 bp insert)
  3. KAH170/KAH015 - deleted hPCD (955 bp insert)
  4. dH2O negative control
  • Dilute 0.5 μL DNA in 10 μL dH2O
  • Add to 50 μL DH5α-turbo
  • Incubate on ice/ 5 min.
  • Spread onto 100 μg/mL Amp plates