User:Karmella Haynes/Notebook/PcTF Genomics/2014/05/19

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  • Cell culture: thaw KAH126 and KAH154

Thaw KAH126 and KAH154

  • Pellet 1mL thawed cells in 5 mL FBS
  • Discard supernatant, leave 100 uL, disrupt the pellet
  • Resuspend in 4 mL 20% FBS/ 1% P/S
  • Grow in T-25 flasks