User:Karmella Haynes/Notebook/PcTF Genomics/2014/04/01

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04/01/14

  • Mammalian cell culturing: Thawed KAH126 & KAH154 lines



Cell culture
Request from Karsten Rippe, Heidelberg

  1. U2OS Flp-in T-REx (parental cell line with no integration)
  2. U2OS Flp-in T-REx with hPCD-TF
  3. U2OS Flp-in T-REx with hPCD-RFP

Notes:

  • Cells thawed on 3/17/14 appear to be forming colonies. It's a miracle!
  • Harvest these and seed cultures at high cell density in 6-well plates (1 well per line)
  • Use 10% FBS, 1% P/S McCoy's 5A