User:Karmella Haynes/Notebook/PcTF Genomics/2011/12/19

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12/19/11

  • ✓ ChIP-seq: Bioanalyzer run; samples submitted to Illumina core (Jason Kramer, Biodesign Institute) on Friday



ChIP-seq bioanalyzer run
> Samples 1-6 (others are empty lanes)
> Run by Jason Kramer
> "2100 expert High Sensitivity DNA Assay DE04103963"

File:KAH121911 bioanalyzer.tif


Sequencing

  • Platform: DNASU's Illumina Hi-Seq, SR flow cell
  • Personnel: Jason Kramer
  • Read length: 1x100 (single end)
  • Fragment length: 200 bp


Lanes (file names)
Note: numbering skips over "4" because this was a control lane

  • Lane 1 = hPc-TF IP
  • Lane 2 = hPc-TF mock
  • Lane 3 = TF IP
  • Lane 5 = TF mock
  • Lane 6 = H3K27me3 IP
  • Lane 7 = H3K27me3 mock