User:Karmella Haynes/Notebook/BioBrick cloning/2013/04/01

Karmella's BioBrick Cloning

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04/01/13 Check MV9 clones MV9 clones Picked colonies from last cloning attempt. Streaked on LB amp agar. Grew up 2 mL cultures overnight and did minipreps. Minipreps Check with S/X digests S present in upstream cloning sites, X inside the new insert Reagent Volume Expected: 1-4. MV9 = 4531, 80 15 μL/lane; 1% agarose; Ladder DNA(plasmid) 3.0 μL 10X buffer 1.5 EcoRI 1.0 PstI 1.0 dH2O 8.5   15 μL --> 37°C/ ~15 min. Result is unclear for #1. Submit for sequencing.