User:Karmella Haynes/Notebook/BioBrick cloning/2012/07/24

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07/24/12

  • Retransform: MV2 (BBa_J176122), MV7 (BBa_J176128)
  • Retransform: ccdB/V0120
  • Retransform: Francesca's plasmids



Retransformations
> MV2 & MV7 for Wisconsin Lutheran College iGEM Team (DH5α Turbo); 200 μg/mL Amp
> ccdB V0120 for lab (One shot ccdB survival; Invitrogen A10460); "
> Francesca's promoter plasmids (DH5α Turbo); "
> Add 0.5 μL DNA to 10 μL dH2O
> FC tubes are dry; wash with 10 μL dH2O

  1. MV2 + DH5α turbo
  2. MV7 + DH5α turbo
  3. FC tube 1 - 2547 promoter + DH5α turbo
  4. FC tube 2 - O2-RFP + DH5α turbo
  5. FC tube 4 - 2547-O2-RFP + DH5α turbo
  6. water + DH5α turbo (neg ctrl)
  7. ccdB/V0120 + One shot ccdB survival
  8. water + One shot ccdB survival