User:Karmella Haynes/Notebook/BioBrick cloning/2011/07/22

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  • ✓ Planning: pSB31 cloning strategy; design adapter; order oligos

Adapter for pSB31 cloning
> Blunt cloning not working well. Use EcoRI/NcoI cloning strategy, add SpeI-EcoRI-NcoI adapter to E/S insert. Two EcoRI sites will allow quick screening of minipreps.
> Oligos:

  1. sense: CTAGTaGAATTCtC (5'-CTAG overhang)
  2. antisense: CATGGaGAATTCtA (5'-CATG overhang)