User:Karmella Haynes/Notebook/BioBrick cloning/2011/07/18

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07/18/11 ✓ Cultures/minipreps: KAH219-224/ pSB31 (4 each) Minipreps > Check with E/P digests Reagent Volume Expected: *pSB31 bands **last frag + 300 1-4. 219/pSB31 1-4 = 4500*, 150*, 444, 1735, 532** 5-8. 220/pSB31 1-4 = 4500*, 150*, 444, 1190** 9-12. 221/pSB31 1-4 = 4500*, 150*, 444, 1490** 13-16. 222/pSB31 1-4 = 4500*, 150*, 636, 1735, 532** 17-20. 223/pSB31 1-4 = 4500*, 150*, 636, 1190** 21-24. 224/pSB31 1-4 = 4500*, 150*, 636, 1490** 13. pSB31 = 4500, 450 15 μL/lane; 1% agarose DNA(plasmid) 2.0 μL 10X buffer 1.5 EcoRI 1.0 PstI 1.0 dH2O 9.5   15 μL --> 37°C/ ~15 min. --> Only KAH220/pSB31 worked. Pick more colonies for other constructs (cultures, o/n).