User:Karmella Haynes/Notebook/BioBrick cloning/2011/07/06

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07/06/11

  • Cultures/minipreps: KAH183-177/MV8 (4)
  • Sequencing order: KAH183-177/MV8 (4), and Daniel's miR-344 sensors



Minipreps
> Check via sequencing


--> Primers: f = DB122, r = BGHR

  1. KH11, 183-177MV8-1, f: 7/7/11 no priming
  2. KH11, 183-177MV8-1, r: 7/7/11 no priming
  3. KH11, 183-177MV8-2, f: 7/7/11 smear; 7/8/11 re-run, good
  4. KH11, 183-177MV8-2, r: 7/7/11 smear; 7/8/11 re-run, good
  5. KH11, 183-177MV8-3, f: 7/7/11 poor quality
  6. KH11, 183-177MV8-3, r: 7/7/11 no priming
  7. KH11, 183-177MV8-4, f: 7/7/11 smear; 7/8/11 re-run, good
  8. KH11, 183-177MV8-4, r: 7/7/11 smear; 7/8/11 re-run, good