User:Karmella Haynes/Notebook/BioBrick cloning/2011/06/02

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06/02/11

  • ✓ Cultures/minipreps/digests: KAH188(2), 190(2), 191(4), 195-198(2 each)



Minipreps
> Check with E/P digests

Reagent Volume Expected:
1,2. KAH188 = 2124
3,4. KAH190 = 2368
5-8. KAH191 = 2223
9,10. KAH195 = 3019
11,12. KAH196 = 3212
13,14. KAH197 = 3263
15,16. KAH198 = 3456
File:KAH 060211 gel1.tif
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.