User:Karmella Haynes/Notebook/BioBrick cloning/2011/02/28

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

02/28/11

  • ✓ Cultures/ minipreps: KAH160, 161, 162, 165, 166, 167, 170 (2 each)



Minipreps
> Check with E/P digests (adds 1326 bp to each predicted fragment size; vector = 4072)

Reagent Volume Expected:
1,2. KAH160/MV1 = 2473
3,4. KAH161/MV1 = 2473
5,6. KAH162/MV1 = 2473
7,8. KAH165/MV1 = 2317
9,10. KAH166/MV1 = 2317
11,12. KAH167/MV1 = 2317
13,14. KAH170/MV1 = 2281
File:KAH 022811 gel1.tif
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.