User:Karmella Haynes/Notebook/BioBrick cloning/2010/07/26

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07/26/10 ✓ Sequencing order: fly mCherry mutagenesis clones Sequencing order (Genewiz standard) --> Forward primer = M13F(-21) (Genewiz) --> Reverse primer = M13R (Genewiz) flymCh-01 F: good flymCh-01 R: good flymCh-02 F: not checked flymCh-02 R: not checked flymCh-03 F: not checked flymCh-03 R: not checked flymCh-04 F: not checked flymCh-04 R: not checked --> Keep clone #1 --> Next, add BioBrick ends via PCR and clone into V0120