User:Karmella Haynes/Notebook/BioBrick cloning/2010/07/23

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

07/23/10

  • ✓ Oligo annealing: microMCS and microMCS2
  • ✓ Cultures/minipreps: 2 mL cultures (4) for mut. flymCh clones



Oligo annealing

  1. microMCS: microMCS 1/ 2
  2. microMCS2: microMCS2 1/ 2
DNA (oligos, 100 μM) up to 18 μL (3 μL ea.)
10x annealing buffer 2.0
dH2O ---
  20 μL --> 100°C (water bath)/ 5 min.; Cool to R.T. overnight