User:Karmella Haynes/Notebook/BioBrick cloning/2010/07/15

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07/15/10

  • ✓ Cultures, minipreps: KHF01-04 (2 mL 5-hour cultures, Turbo)



Minipreps
> Check with E/P digests

Reagent Volume Expected:
1,2. KHF01 = 21
3,4. KHF02 = 714
5,6. KHF03 = 711
7,8. KHF04 = 714
File:KAH 071510 gel2.tif
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.

--> Send 1, 3-8 to Genewiz for sequencing (tomorrow)