User:Karmella Haynes/Notebook/BioBrick cloning/2010/06/05

From OpenWetWare
Jump to navigationJump to search
Project name Main project page
Previous entry      Next entry

06/05/10

  • ✓ Order primers: for Hygro amplification

Order Primers

> PCR insert: Hygromycin R gene from V0200 (new oligos with longer ends):

  1. Hygro f2 5'-tagcagacatagacaga gatgaggatc atg AAAAAGCCTGAACTCACCGC (has BsaBI and Met)
  2. Hygro r2 5'-cagccgaatcgcatcgacgcgtac ttcgaa CTATTCCTTTGCCCTCGGAC (has BstBI)



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Karmella_Haynes/Notebook/BioBrick_cloning/2010/06/05&oldid=1004486"