User:Karmella Haynes/Notebook/BioBrick cloning/2010/05/19

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

05/19/10

  • ✓ Cultures: MV7 (4x 2mL cultures, 6 hours)
  • ✓ Minipreps: MV7



Minipreps
> Check with PstI digests

Reagent Volume Expected:
1-4. MV7 = 3386, 1356
File:KAH 052110 gel1.tif
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
PstI 1.0
dH2O 10.5
  15 μL --> 37°C/ ~15 min.


--> Success! Use for miR sensor constructs.