User:Karmella Haynes/Notebook/BioBrick cloning/2010/04/19

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04/19/10

  • ✓ Sequencing order: KAH154-159/MV2; KAH96, 142, 146, 147/V0201



Sequencing order (Genewiz, standard, 4/20/10)
--> Mix-up: Sequence reactions were actually set up (by me) in this order: 154, 155, 156, 158, 159, 157

  1. KAH154/MV2 DDB122: good
  2. KAH154/MV2 DDB123: good
  3. KAH155/MV2 DDB122: good
  4. KAH155/MV2 DDB123: good
  5. KAH156/MV2 DDB122: fshPcCD! (should be fly)
  6. KAH156/MV2 DDB123: fshPcCD! (should be fly)
  7. KAH157/MV2 (actually 158) DDB122: good
  8. KAH157/MV2 (actually 158) DDB123: good
  9. KAH158/MV2 (actually 159) DDB122: good
  10. KAH158/MV2 (actually 159) DDB123: good
  11. KAH159/MV2 (actually 157) DDB122: flyPcCD! (should be fsh)
  12. KAH159/MV2 (actually 157) DDB123: flyPcCD! (should be fsh)
  13. KAH96/MV2 3t3: good
  14. KAH96/MV2 BGHR: good
  15. KAH142/MV2 3t3: good
  16. KAH142/MV2 BGHR: good
  17. KAH146/MV2 3t3: looks like 147
  18. KAH146/MV2 BGHR: good
  19. KAH147/MV2 3t3: looks like 146
  20. KAH147/MV2 BGHR: good


--> Tomorrow: check KAH156, 157/MV2 with AvaI digest for correct i.d.; also XbaI digests for KAH94, 142, 146, 147/V0201