User:Karmella Haynes/Notebook/BioBrick cloning/2010/02/23

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02/23/10 ✓ Minipreps: DPRE (4) ✓ Sequencing order: DPRE clones Minipreps > Check with E/S and E/P digests Reagent Volume Expected: 1,3,5,7. DPRE (E/S) = 1740 2,4,6,8. DPRE (E/P) = 1001, 662, 77 15 μL/lane; 1% agarose DNA(plasmid) 2.0 μL 10X buffer 1.5 EcoRI 1.0 PstI 1.0 dH2O 9.5   15 μL --> 37°C/ ~15 min. --> Send all 4 off for sequencing. Sequencing --> Genewiz standard sequencing service DPRE-01 P0001 (fwd): good (keep) DPRE-01 P0002 (rev): good DPRE-02 P0001 (fwd): good DPRE-02 P0002 (rev): good DPRE-03 P0001 (fwd): good DPRE-03 P0002 (rev): good DPRE-04 P0001 (fwd): good DPRE-04 P0002 (rev): good