User:Karmella Haynes/Notebook/BioBrick cloning/2010/02/16

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02/16/10

  • ✓ Order primers: for BioBrick cloning of DPRE (from CJW)

Primer order
> Note: part contains 2 PstI sites > Direct E/S cloning upstream of YFP reporter (ignore PstI's)

  1. BBp_DPRE f: 5'-gaattcgcggccgcatctagaCCGAAGAGAAAGGACGCTAA
  2. BBs_DPRE r: 5'-ctgcagcggccgctactagtAACCGTGCGGACAGACAGAG

> PstI mutagenesis primers (make an official BioBrick; to be done in parallel)

  1. mut_DPRE 1: 5'-GAAATGCTcCAGTCCAAGCGGG
  2. mut_DPRE 2: 5'-GCGCCACTcCAGATGGCTCACC