User:Karmella Haynes/Notebook/BioBrick cloning/2010/02/08

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02/08/10

  • ✓ Minipreps: KAH80 (2)

Minipreps
> Check with E/P digests (was done by Jason L. to replace plasmid DNA used earlier)

Reagent Volume Expected:
1,2. KAH80 = 1474
File:KAH 020810 gel1.tif
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.

--> Combine preps, store @ -20°C