User:Karmella Haynes/Notebook/BioBrick cloning/2010/01/26

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01/26/10

  • ✓ Cultures: KAH140, 141/ V0201 (2 each)
  • ✓ Minipreps: KAH94/ JDS48, 49 (2 each)

Minipreps
> Check with BglII and PstI
> Include KAH94 and JDS48 as controls

Reagent Volume 1,2. KAH94/JDS48 BglII
3,4. KAH94/JDS49 BglII
5. KAH94 BglII
6. JDS48 BglII

7,8. KAH94/JDS48 PstI
9,10. KAH94/JDS49 PstI
11. KAH94 PstI
12. JDS48 PstI
File:KAH 012610 gel1.tif
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
enzyme 1.0
dH2O 10.5
  15 μL --> 37°C/ ~15 min.

--> Clones were false positives. Discontinue building these constructs. Instead use miR sensing device separately from Joe's luciferase plasmid (will make stable lines).