User:Karmella Haynes/Notebook/BioBrick cloning/2010/01/19

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01/19/10

  • ✓ Minipreps: KAH94, 95/JDS49 (6 each); KAH94, 95/JDS48 (6 each)
  • ✓ Cultures: KAH139, 140, 141 (2 each)
  • ✓ Cultures: KAH118/MV2 (12)

Minipreps
> Check with X/P digests

Reagent Volume Expected:
Empty: 3554, 2053, 1871
Fwd ins: 5500, 3039, 2053, 1871
Rev ins: 8039, 2053, 1871, 515
1-6. KAH94/JDS49
7-12. KAH95/JDS49
13-18. KAH94/JDS48
19-24. KAH95/JDS48
File:KAH 011910 gel1.tif
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
XbaI 1.0
PstI 1.0
dH2O 9.5
  15 μL

--> 37°C/ ~15 min.
--> Not sure about results. Check plasmids via PCR (RFP primers) tomorrow.