User:Karmella Haynes/Notebook/BioBrick cloning/2010/01/15

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01/15/10

  • ✓ Minipreps: KAH137, 138

Minipreps
> Check with E/P digests

Reagent Volume Expected:
V0120 = 3200
1,2. KAH137 = 2393
3,4. KAH138 = 1981
File:KAH 011510 gel1.tif
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.

--> Pick 3 more col's for each construct