User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/28

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12/28/09

  • ✓ Cultures: KAH136(2), 137(4), 138(4); retransformations KAH93(2), JDS42(2), JDS43(2)
  • ✓ Oligo annealing: BglII adapters for RFP-rep inserts into JDS42 & 43

Oligo annealing

  1. Ad BglII-EcoRI
  2. Ad PstI-BglII
DNA (oligos, 100 μM) up to 18 μL (3 μL ea.)
10x annealing buffer 2.0
dH2O ---
  20 μL --> 100°C (water bath)/ 5 min.; Cool to R.T. overnight