User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/19

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12/19/09

  • ✓ Minipreps: KAH117(3), 118(4), 119(1)

Minipreps
> Check with E/P digests

Reagent Volume Expected:
1-3. KAH117 = 7758
4-7. KAH118 = 7930
8. KAH119 = 8002
File:KAH 121909 gel1.tif
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.