User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/15

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12/15/09

  • ✓ Cultures: KAH126-13/MV2 (2 each)
  • ✓ Minipreps: KAH123-124 (3 each)
  • ✓ Cultures: KAH123, 125 (6 each)

Minipreps
> Check with E/P digests

Reagent Volume Expected:
1,2. KAH123 = 8002
3,4. KAH124 = 8174
5,6. KAH125 = 8246
File:KAH 121509 gel1.tif
15 μL/lane; 1% agarose -->
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.