User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/06

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12/06/09

  • ✓ Minipreps: KAH95/MV2(4)

Minipreps
> Check with E/P/ScaI digests

Reagent Volume Expected:
1-4. KAH95/MV2 = 5083 		E/P/ScaI digest 12/06/09
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
ScaI 1.0
dH2O 8.5
  15 μL --> 37°C/ ~15 min.



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