User:Karmella Haynes/Notebook/BioBrick cloning/2009/11/26

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11/26/09

  • Minipreps: KAH96(2), 97(2), 98(2), 011(6)

Minipreps
> Check with E/P digests

Reagent Vol. (1-6) Vol. (7-12) Expected:
1,2. KAH96 = 2825
3,4. KAH97 = 4407
5,6. KAH98 = 5989
7-12. KAH011 = 64
File:KAH 112609 gel1.tif
15 μL/lane; 1% agarose
Need to re-clone KAH011.
DNA(plasmid) 2.0 4.0
10X buffer 1.5 1.5
EcoRI 0.5 0.5
PstI 0.5 0.5
dH2O 10.5 8.5
  15 μL 15 μL --> 37°C/ ~15 min.