User:Karmella Haynes/Notebook/ASU courses/2012/01/23

BME194 Syn Bio Demo

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1/23/12 Pour plates: Amp/Kan (100/50 μg/mL) and Kan (50 μg/mL) agar plates Transform: Quick and dirty transformation of plasmids from Francesca Francesca's GFP induction plasmids > Use NEB10B competent cells (DH5α turbo has Lac repressor), 50 μL cells each... Amp: Reporter wih O2 lac operator on pSB1A2 (see paper) Amp: Reporter wih O1 lac operator on pSB1A2 (see paper) Amp: Reporter wih OSym lac operator on pSB1A2 (see paper) Kan: Negative feedback based LacI generator with O2 lac operator (the weakest) on pSB3K3 (see paper) Amp: no DNA Kan: no DNA Ref: Ceroni et al., JBE, 2010 Will mini prep later and co-transform Reporters with LacI generator