User:Karmella Haynes/Notebook/ASU courses/2012/01/31

ASU Courses

Main project page Previous entry      Next entry

01/31/12 (BME 194) Section C class BME 194 Synthetic Bio section > 1 set of cuvettes = Cuvette 1: O2-GFP (positive control + 4.0 mM IPTG) Cuvette 2: O#-GFP/ LacI (0.0 IPTG) Cuvette 3: O#-GFP/ LacI (0.25 IPTG) Cuvette 4: O#-GFP/ LacI (0.5 IPTG) Cuvette 5: O#-GFP/ LacI (1.0 IPTG) Cuvette 6: O#-GFP/ LacI (2.0 IPTG) Cuvette 7: O#-GFP/ LacI (4.0 IPTG) Cuvette 8: LacI (4.0 IPTG) Exercise 1: GFP measurement: Vikram's spectral analyzer; fill 2 mL cuvettes w/ 1 mL culture Section A (Tuesday afternoon, 3 pm) Switch A (O2-GFP) - 2 sets Switch B (O1-GFP) - 3 sets Switch C (Osym-GFP) - 3 sets Students record data on worksheet #1 Exercise 2: Students also measure OD600 on spectrophotometer Exercise 3: Students take pictures of streaked agar plates using Vikram's web cam with filters