User:Karmella Haynes/Notebook/ASU courses/2012/01/31

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01/31/12

  • (BME 194) Section C class



BME 194 Synthetic Bio section
> 1 set of cuvettes =

  1. Cuvette 1: O2-GFP (positive control + 4.0 mM IPTG)
  2. Cuvette 2: O#-GFP/ LacI (0.0 IPTG)
  3. Cuvette 3: O#-GFP/ LacI (0.25 IPTG)
  4. Cuvette 4: O#-GFP/ LacI (0.5 IPTG)
  5. Cuvette 5: O#-GFP/ LacI (1.0 IPTG)
  6. Cuvette 6: O#-GFP/ LacI (2.0 IPTG)
  7. Cuvette 7: O#-GFP/ LacI (4.0 IPTG)
  8. Cuvette 8: LacI (4.0 IPTG)

Exercise 1: GFP measurement: Vikram's spectral analyzer; fill 2 mL cuvettes w/ 1 mL culture

  • Section A (Tuesday afternoon, 3 pm)
    • Switch A (O2-GFP) - 2 sets
    • Switch B (O1-GFP) - 3 sets
    • Switch C (Osym-GFP) - 3 sets

Students record data on worksheet #1


Exercise 2: Students also measure OD600 on spectrophotometer

Exercise 3: Students take pictures of streaked agar plates using Vikram's web cam with filters