User:Karmella Haynes/Notebook/ASU courses/2012/01/29

ASU Courses

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01/29/12 (BME 194) Experimental cultures Experimental cultures for Monday sections > Innoculate overnight cultures w/ 10 uL starter culture (20 tubes, 10 mL each)   Medium IPTG (mM) O2-GFP Amp           4.0 (2x) O2-GFP/ LacI Amp/ Kan 0.0 0.25 0.5 1.0 2.0 4.0 O1-GFP/ LacI Amp/ Kan 0.0 0.25 0.5 1.0 2.0 4.0 Osym-GFP/ LacI Amp/ Kan 0.0 0.25 0.5 1.0 2.0 4.0 LacI Kan           4.0 (2x) Banana induction: 5 mL each Banana, no IAA Banana, + 5 uL IAA Streak plates: (amp) for RFP1, GFP4, Banana > Sunday night: make 15 (will recycle for both class days)