User:Karmella Haynes/Notebook/ASU courses/2012/01/29

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01/29/12

  • (BME 194) Experimental cultures



Experimental cultures for Monday sections
> Innoculate overnight cultures w/ 10 uL starter culture (20 tubes, 10 mL each)

  Medium IPTG (mM)
O2-GFP Amp           4.0 (2x)
O2-GFP/ LacI Amp/ Kan 0.0 0.25 0.5 1.0 2.0 4.0
O1-GFP/ LacI Amp/ Kan 0.0 0.25 0.5 1.0 2.0 4.0
Osym-GFP/ LacI Amp/ Kan 0.0 0.25 0.5 1.0 2.0 4.0
LacI Kan           4.0 (2x)


Banana induction: 5 mL each

  1. Banana, no IAA
  2. Banana, + 5 uL IAA


Streak plates: (amp) for RFP1, GFP4, Banana
> Sunday night: make 15 (will recycle for both class days)