1/25/12
- Minipreps: O2-GFP, O1-GFP, Osym-GFP, LacI-O2 for minipreps (5 mL)
- Transformations: co-transform reporter/ LacI
Minipreps
> Measure DNA concentration using the Take3 plate (Sarah's Odyssey system)
Sample |
OD 260 |
260/280 |
ng/μL
|
1. O2-GFP |
1.613 |
2.208 |
80.665
|
2. O1-GFP |
1.911 |
2.151 |
95.538
|
3. Osym-GFP |
1.019 |
2.154 |
50.961
|
4. LacI-O2 |
1.189 |
2.214 |
59.459
|
Transformation
> Use 25 ng of each plasmid
Plasmid 1 |
μL |
Plasmid 2 |
μL
|
1. O2-GFP |
0.3 |
LacI-O2 |
0.4
|
2. O1-GFP |
0.3 |
LacI-O2 |
0.4
|
3. Osym-GFP |
0.5 |
LacI-O2 |
0.4
|
> Add 50 μL NEB10B cells (quick and dirty transformation); plate on 100 μg/mL Amp + 50 μg/mL Kan LB agar
|