- Continue preparing polyvinyl alcohol microspheres for experimental analysis
- Dilute Rhodamine 6G dye solution down to 1μM concentration for prepared microspheres
- Prepare DSC samples for polyvinyl alcohol microspheres created
Microsphere PVOH 146K 110% CEC Laponite w/ DMHXLBR Information
|Preparation of 110% Laponite Microspheres using PVOH MW 146,000-186,000
|PVOH 146K added(g)
|110% Laponite clay added (g)
|Cellulose Acetate Butyrate added (g)
|Glutaraldehyde added (mL)
|H2SO4 added (mL)
Rhodamine 6G Dye Dilution Calculations
1μM Rhodamine 6G Dye Concentration
M1V1 = M2V2
1μM (RG6)x 25mL = (92μM)V2 V2 = 271.74μL
DSC Analysis: Exchanged Clays & PVOH 146K Microspheres
DSC preparation and protocal involving instrumentation and usage can be found on 9/17/12 entry.
||Sample Mass (mg)
||Pan / Lid Mass (mg)
|50% CEC NaMT w/ Bu3HdP+
|110% CEC NaMT w/ DMHXLBR
|110% CEC Laponite w/ DMHXLBR
|90:10 PVOH 146K Laponite Dust
|90:10 PVOH 146K Laponite Medium Clumps
|90:10 PVOH 146K Laponite Large Clumps
- After preparing the microspheres from the PVOH 146K Laponite, many of the spheres were not spherical. Large clumps of all sizes were produced rather than microspheres.
- The microspheres prepared from PVOH 146K Laponite were rigid white crystalline structures with stacked layers.
- Due to large sizes of the microspheres, they had to be ground up using a mortar and pestle before adding the 1μM rhodamine 6G dye solution.
- Also DSC was taken of the different sizes of the microspheres to determine if the structure, function, and capability of the microspheres had changed due to extensive clumping.
- When going through the process of preparing new microspheres from PVOH 146K 110% CEC Laponite w/ DMHXLBR, due to slowed vacuum filtration the sample was placed in a vial with the last solvent used for cleaning, methanol.
- When freezing the new microsphere sample in liquid nitrogen, PVOH 146K 110% CEC Laponite w/ DMHXLBR, it was frozen in a medium of methanol and vacuum filtrated to remove the methanol residue.