User:Jose Fabricio Lopez/Notebook/Logbook/2011/08/20

iGEM 2011

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Entry title See Gel from yesterday PCRs Gel Lane 1 Ladder Lanes 2-4 Vladimir´s PCR Lane 5 Insert PCR Lane 6 Insert PCR Lane 7 Backbone PCR Lane 8 Backbone PCR Lane 9 Insert Control Lane 10 Backbone Control Lane 11 Ladder RepC PCR Reagent Extraction Primordial Plasmid(Given by PhD.Miguel Cevallos Control DNA from Plate1A7 10x PCR buffer Taq Platinum 5μL 5μL 5μL 8mM dNTPs mix 5μL 5μL 5μL 50mM MgCl2 1.5μL 1.5μL 1.5μL Upper_repC primer 5μM 2μL 2μL 2μL Low_repC primer 5μM 2μL 2μL 2μL Template repC region 1μL 1μL 1μL Platinum Taq DNA-pol 0.5μL 0.5μL 0.5μL H2O 33.5μL 33.5μL 33.5μL Total 50μL 50μL 50μL